acquired 4th report from 48 hours after 4.3 and right down to 1.7, implied half-life of 36 hours from that beginning stage. Is sensible and according to chart posted above (or different thread?) that half-life longer from decrease beginning worth.
I’m somewhat unclear what the best values for Tmax and trough stage earlier than subsequent dose I needs to be aiming for. I’ll must dig round on this thread and right here to research. I prob noticed some hints that didn’t sink in earlier than I began my testing.
Or I suppose I can ask Dr. Inexperienced.
Oh, I forgot to say, I additionally take CBD and Theracumin(Curcumin), and iirc the previous ought to inhibit Rapa metabolization and the latter appears to extend it and enormously decrease AUC: Turmeric Linked to Liver Injury, Lower in Rapamycin Bioavailability. (Although confusingly I’ve seen it each methods, that Curcumin each inhibits and prompts the related CYP pathway. I’ll put up within the thread about that…)
[side note on CBD. I took it to help sleep, but it cured my Morton’s Neuroma (nerve pain in foot) immediately! I’d had annoying pain in the morning literally every single day for over a year, and a Dr rec’d CDB for sleep, so I gave it a try, and the next morning after being awake for a while I realized that my foot hadn’t hurt at all. So I’ve been taking it every since and the pain hasn’t returned. And it can’t be placebo, because I took it for sleep and it never occurred to me it would help with foot pain…]
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Sure – you have to be somewhat unclear… no person actually is aware of for positive. On the Tmax stage, within the NIA ITP mouse research, typically the upper the dose, the higher the lifespan improve. In a single research ( of the 45+ research of rapamycin in mice) they did lastly discover an higher dose of rapamycin that not elevated lifespan in these feminine mice. It was a particularly excessive dose although.
On trough ranges, individuals appear to focus on beneath 1ng/ML per right here: The best way to get a Rapamycin (sirolimus) Blood Degree Take a look at however once more, we don’t have absolute numbers right here.
I ought to be aware that in mice there’s a vital dose / response distinction between male mice and feminine mice. This distinction just isn’t seen in people.
Most of individuals at present utilizing rapamycin appear to be (in mouse equal dosing) within the low ranges examined (be aware: mice have been dosed rapamycin of their meals, each day, so that’s one other distinction – vs. us utilizing rapamycin dosed as soon as weekly or so).
Listed here are the Nationwide Institutes on Growing older Outcomes from their rapamycin research (these are one of the best rapamycin research):
| Sirolimus Dose |
mg/kg/day Dose |
Blood/Sirolimus Degree |
Male Median LS Enhance | Feminine Median LS Enhance |
|---|---|---|---|---|
| 4.7ppm | ∼2.24 | 3 to 4 ng/mL | 3% | 16% |
| 14ppm | ~6.67 | 9-16 ng/mL | 13% | 21% |
| 42ppm | ~20 | 23-80 ng/mL | 23% | 26% |
Listed here are outcomes from all the upper dose research I might discover:
| Sirolimus Dose |
Mouse mg/kg/day Dose |
Mouse: Blood/Sirolimus Degree |
Human mg/kg/day Dose |
Dose for 60kg Human | Day by day Dose adjusted for longer half-life (/4) |
|---|---|---|---|---|---|
| 4.7ppm | ∼2.24 | 3 to 4 ng/mL | 0.182 mg/kg | 10.92 mg | 2.73 mg |
| 14ppm | ~6.67 | 9-16 ng/mL | 0.542 mg/kg | 32.54 mg | 8.135 mg |
| 42ppm | ~20 | 23-80 ng/mL | 1.626 mg/kg | 97.56 mg | 24.39 mg |
| 126ppm | ~60 | 4.878 mg/kg | 292.68 mg | 73.17 mg | |
| 378ppm | ~180 | 45 to 1800 ng/mL | 14.634 mg/kg | 878.04 mg | 218 mg |
Based mostly on the FDA animal to human dosing conversion information right here.
Be aware: ½ life for sirolimus in mice is approx. 15 hours, vs. approx. 62 hours in people. So, mice metabolize sirolimus roughly 4 occasions sooner than people.
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Improbable, thanks!
You simply saved me numerous hours I had meant to spend to gather and convert (after determining methods to convert) that knowledge!
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Thats what the location is for… everybody shares the work they do do, and the info they collect, so that everybody doesn’t must do all of it themselves!
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Nicely, simply acquired my outcomes again after 10mg + contemporary grapefruit juice (and I ate pulp for good measure) + swig of olive oil: solely 15.6
That’s in comparison with earlier 10 mg dose on empty abdomen which led to 11.2
Each after 90 minutes. Possibly I want to attend longer to check?
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I’m unsure ready longer will make a distinction I wait simply an hour and a 1/2 and get 6 to 7 occasions the rise.
Everyone seems to be totally different.
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However good for you for taking the measurements.
That is the one approach that you just’re going to know the way your physique is responding to GFJ and your dose of rapamycin.
Right here’s my consequence. That is about 90 minutes after taking 2 1mg Biocon tablets along with 2 1mg Zydus tablets with half a small crimson grapefruit, 2Tbs of EVOO, washed down with a small quantity of pasteurized crimson grapefruit juice. The take a look at consequence additionally places to relaxation the notion that the Indian product is faux or ineffective.
Sirolimus (Rapamune), Blood
Take a look at Present Consequence
Sirolimus, Blood 31.8 ng/mL Reference interval 3.0-20.0
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Is Marek Well being nonetheless providing the sirolimus take a look at? Hyperlink didn’t work for me
If not, who has the most cost effective take a look at?
You’re proper – doesn’t work on cell for me, however works on PC
FWIW, there’s a small test 
that’s troublesome to see that should be checked to course of.
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They modified their web site, so previous direct hyperlink doesn’t work, however this one appears to:
If that doesn’t you’ll be able to go to “Lab Builder” and seek for take a look at
